US EPA

1149676 

Journal Article 

High-Performance Liquid Chromatographic Separation of Indandione Rodenticides 

Houglum, JE; Larson, RD; Neal, RM 

1989 

1 

Journal of Chromatography
ISSN: 0021-9673 

NIOSH/00194116 

481 

458-460 

A high performance liquid chromatographic (HPLC) method for separation of four indandione anticoagulant rodenticide agents was described. Chlorophacinone (3691358), diphacinone (82666), pindone (83261), and valone (83283) were dissolved in methanol, diluted with methanol and/or acetonitrile, and further diluted with phosphoric-acid to a concentration of 2.5 micrograms per milliliter. An HPLC column was used with the mobile phase consisting of acetonitrile and phosphoric-acid. Rodenticide was applied to ground corn to yield concentrations of 0.005 percent chlorophacinone or diphacinone, or 0.025 percent pindone or valone. Spiked bait was extracted with acetonitrile or methanol and acetonitrile, diluted with 0.2 percent phosphoric-acid and analyzed. Capacity factors were 2.5, 2.8, 4.6, and 5.6 for valone, pindone, diphacinone, and chlorophacinone, respectively. Correlation of response was high (0.997 to 0.999) for all four agents. Mean recovery ranged from 83.3 to 96.9 percent for chlorophacinone and valone, respectively; coefficients of variation ranged from 0.6 to 3.8 percent for valone and diphacinone, respectively. Recovery of pindone and valone was enhanced by adding methanol to the extraction solvent. The authors conclude that this method is useful for identifying indandione in mislabeled or unknown samples. 

DCN-189504; Analytical chemistry; Anticoagulants; Chemical analysis; Chromatographic analysis; Laboratory techniques; Rodenticides; Sample preparation; Toxicology