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1155495 
Journal Article 
Preparation and characterization of paclitaxel from plant cell culture 
Gi, US; Min, B; Lee, JH; Kim, JH 
2004 
1 
Korean Journal of Chemical Engineering
ISSN: 0256-1115
EISSN: 1975-7220 
Korean Institute of Chemical Engineers 
SEOUL 
21 
4 
816-820 
English 
WOS:000223325700011 
The solvent treatment of paclitaxel is a convenient method for controlling the morphologies of paclitaxel. Amorphous paclitaxel was simply made by dissolving paclitaxel in methylene chloride/methanol (98/2, v/v) and in relatively non-polar solvents (t-butyl methyl ether, pentane, acetonitrfle/hexane (1/2, v/v), methylene chloride, chloroform). On the other hand, dihydrated paclitaxel (paclitaxel(.)2H(2)O) was made by dissolving paclitaxel in a special polar solvent containing a small amount of water. However, when we used only methanol, we got mixed morphologies of paclitaxel made of both the dihydrated and amorphous forms. Their physicochemical properties were investigated by X-ray powder diffraction (XRPD), differential scanning calorimetry (DSC), and thermo gravimetric analysis (TGA). The initial water content of amorphous paclitaxel and dihydrated paclitaxel was determined for 0.65 wt% and 5.85 wt%, respectively. The hygroscopic property of dihydrated paclitaxel was very changeable in A given humidity (15, 60, 95 RH%) during storage. Dissolution profiles for pactitaxel showed that amorphous pactitaxel measured the highest solubility in water and its solubility held most stable during the measurements. The residual solvent could be reduced to the maximum allowed value (600 ppm for methylene chloride, 3,000 ppm for methanol) of guidance for the International Conference on Harmonization (ICH) by spray drying. 
amorphous paclitaxel; dihydrated paclitaxel; X-ray powder diffraction; thermo gravitnetric analysis; physicochemical property; spray drying