US EPA

1158200 

Journal Article 

Preparation of FAME from sterol esters 

Ichihara, K; Yamaguchi, C; Nishijima, H; Saito, K 

2003 

Yes 

Journal of the American Oil Chemists' Society
ISSN: 0003-021X
EISSN: 1558-9331 

SPRINGER 

NEW YORK 

80 

8 

833-834 

WOS:000184905200017 

CH3ONa, the formation of FFA was negligible with this dried solution (Fig. 2A). The yield of methyl oleate was higher than 94%, indicating that methyl propionate served as a good substitute for benzene or diethyl ether, which had been used earlier (4,5). Wax, i.e., long-chain FA esters of higher alcohols, and sterol esters are also resistant to methanolysis under the conditions for glycerolipids (2). Figure 213 shows that the same procedure as the one proposed for the methanolysis of sterol esters is applicable for wax. The reaction of wax was faster than that of sterol esters and was complete within 40 min. Thus, the combination of methyl propionate and the methanolic NaOH solution dried with Molecular Sieves 3A was an efficient reagent for the methanolysis of sterol esters or wax.To prevent possible hazards, we would like to recommend the use of this safe solvent system for the preparation of FAME from sterol esters, wax, or lipid samples containing both glycerolipids and sterol esters, such as blood lipids. ne procedure utilizes neither benzene nor diethyl ether. Methanol, methyl propionate, and hexane are used as the organic solvents. Methanolic CH3ONa is commonly prepared from methanol and Na metal, which may cause a fire, whereas the dried methanolic NaOH solution can be more safely and easily prepared than methanolic CH3ONa.