US EPA

1165610 

Journal Article 

Production of biodiesel fuel from triglycerides and alcohol using immobilized lipase 

Iso, M; Chen, BX; Eguchi, M; Kudo, T; Shrestha, S 

2001 

Yes 

Journal of Molecular Catalysis B: Enzymatic
ISSN: 1381-1177 

Elsevier 

16 

1 

53-58 

10.1016/S1381-1177(01)00045-5 

WOS:000172018400007 

https://linkinghub.elsevier.com/retrieve/pii/S1381117701000455
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Transesterification reaction was performed using triglycerides and short-chain alcohol by immobilized lipase in non-aqueous conditions. The long-chain fatty acid ester, which is the product of this reaction, can be used as a diesel fuel that does not produce sulfur oxide and minimize the soot particulate. Immobilized Pseudomonas fluorescens lipase showed the highest activity in this reaction. Immobilization of lipase was carried out using porous kaolinite particle as a carrier. When methanol and ethanol were used as alcohol, organic solvent like 1,4-dioxane was required. The reaction could be performed in absence of solvent when 1-propanol and 1-butanol were used as short-chain alcohol. The activity of immobilized lipase was highly increased in comparison with free lipase because its activity sites became more effective. Immobilized enzyme could be repeatedly used without troublesome method of separation and the decrease in its activity was not largely observed. (C) 2001 Elsevier Science B.V. All rights reserved. 

enzyme biocatalysis; lipase; transesterification; Immobilized enzyme; biodiesel fuel