US EPA

1170533 

Journal Article 

Spectrophotometric determination of iron(II) in seawater at nanomolar concentrations 

King, DW; Lin, J; Kester, DR 

1991 

Yes 

Analytica Chimica Acta
ISSN: 0003-2670
EISSN: 1873-4324 

BIOSIS/91/25431 

247 

1 

125-132 

English 

BIOSIS COPYRIGHT: BIOL ABS. Natural Fe(II) concentrations in seawater have been determined by using the colorimeter reagent ferrozine (3-(2-pyridyl)-5,6-bis(4-phenylsulfonic acid)-1,2,4-triazine). The ferrozine was adsorbed on a C18 Sep-Pak cartridge. Seawater samples ranging in volume from 50 to 400 ml were passed through the Sep-Pak with the Fe(II) being retained as the colored Fe(II)-ferrozine complex. The complex was extracted from the Sep-Pak column with 10 ml of methanol. Up to a 40-fold preconcentration of Fe(II) was possible using this method. The molar absorptivity of the Fe(II)-ferrozine complex in methanol was 28.1m the Sep-Pak was 91%. The detection limit for Fe(II) in seawater was 0.6 nmol l-1. The precision over the range 0.6-185 nmol l-1 was 2% or 0.2 nmol l-1, whichever is the larger. Copper(I) interference was minimized by complexing Cu(I) with neocuproine added to the methanol Sep-Pak effluent. 

Ecology; Biochemical Methods-Minerals; Biochemical Studies-Minerals; Biophysics-General Biophysical Techniques; Public Health: Environmental Health-Air