US EPA

1182571 

Journal Article 

Multiresidual method for determination of the uracil herbicides in plant samples by GC/NPD 

Tekel, J; Tahotna, S; Pecnikova, K; Holla, M; Vaverkova, S 

1998 

1 

Deutsche Lebensmittel-Rundschau
ISSN: 0012-0413 

94 

10 

334-336 

English 

WOS:000076492700002 

GC method is described for the simultaneous determination or the uracil herbicide residues in some medicinal plants, spinach and strawberries. The uracil herbicide (bromacil, lenacil and terbacil) residues were extracted into acetone or acetone-water (95:5, v/v). After evaporation of acetone from acetone-water extract the residues were dissolved in water-methanol (5:1. v/v). Cyclohexane was used for removal of the non-polar co-extractives in sample matrix. After separation of cyclohexane phase the uracil herbicide residues were extracted into dichloromethane. The extract was purified on a Florisil® column, residues were eluted with dichloromethane-acetone (95:5, v/v) and analysed by capillary GC with selective detector (NPD) using a DB-17 column. A minimum recovery of 70% was attained on the contamination levels of 0.02-0.2 mg/kg, the limit of detection was 0.005-0.01 mg/kg (S/N = 3). The lenacil residue content in dry plant of M.piperita L. and M.officinalis L. from the micro-scale experiment was in the range <0.02 to 0.05 mg/kg. Analysis of actual samples of spinach and strawberries produced by farm in Slovakia did not show the presence of the uracil herbicide residues (the content was below the 0.01 mg/kg).