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1190619 
Journal Article 
HPLC determination of rhodamine B (CI 45170) in cosmetic products 
Gagliardi, L; Deorsi, D; Cavazzutti, G; Multari, G; Tonelli, D 
1996 
Yes 
Chromatographia
ISSN: 0009-5893
EISSN: 1612-1112 
43 
1-2 
76-78 
English 
WOS:A1996VB64100012 
A method is proposed for the extraction, separation, identification and quantitative measurement of rhodamine B in cosmetics samples The colour is extracted with a solution of orthophosphoric acid in either water or DMF (depending on the type of cosmetic) adsorbed on a polyamide column, and then eluted with water and methanol. Further clean-up of the extract is performed by means of a Bond Elut C-18 cartridge, from which rhodamine B is eluted with methanol-water (8:2). The final eluate is examined by HPLC. Chromatography is performed on a C-18 column with a gradient elution from 50:50 to 70:30 of acetonitrile-water, containing 0.1 M sodium perchlorate, in 15 min. A diode array detector enables peak purity analysis. The method gives recovery values of approximately 90%, or better, and the reproducibility is within 4%. 
column liquid chromatography; rhodamine B in cosmetics 
IRIS
• Methanol (Non-Cancer)
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