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HERO ID
1234059
Reference Type
Journal Article
Title
Zinc PVA versus potassium dichromate for preservation of microsporidian spores of human origin
Author(s)
El-Taweel, HA; Tolba, MM; Sadaka, HA; El-Zawawy, LA; Osman, MM
Year
2012
Is Peer Reviewed?
1
Journal
Parasitology Research
ISSN:
0932-0113
EISSN:
1432-1955
Volume
111
Issue
2
Page Numbers
689-694
Language
English
PMID
22427024
DOI
10.1007/s00436-012-2888-8
Abstract
Microsporidia are emerging opportunistic parasites. Preservation of the biological properties of microsporidian spores is often required in research work. The present study compared two preservatives; zinc polyvinyl alcohol (zinc PVA) and potassium dichromate solutions for preservation of microsporidian spores separated from human faecal samples. After 0, 1, 2 and 4 months of storage, morphological features and staining characters of the spores were assessed by light microscopy in modified trichrome-stained smears and their viability percentages were calculated using acridine orange/ethidium bromide mixture. Also, spore infectivity was evaluated by faecal spore shedding and intestinal spore load in mice orally inoculated with the preserved spores. Results revealed that morphological features, staining characters and viability of the spores were maintained in both solutions throughout the study period. Spore infectivity was completely preserved in zinc PVA solution but showed significant reduction in potassium dichromate solution at the fourth month of the preservation duration.
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IRIS
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Chromium VI
Considered
Excluded
Other Not Pertinent
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