Effect of chromate ion on the membrane of established human cells as measured by uptake of a permeant lipophilic cation | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

1234834

Reference Type

Journal Article

Title

Effect of chromate ion on the membrane of established human cells as measured by uptake of a permeant lipophilic cation

Author(s)

Brun, EC; White, LR; Eik-Nes, KB

Year

1987

Is Peer Reviewed?

Journal

Toxicology Letters
ISSN: 0378-4274
EISSN: 1879-3169

Volume

Issue

2-3

Page Numbers

253-259

Language

English

PMID

3824414

Abstract

Cells from the established human cell line NHIK3025 concentrate the permeant lipophilic cation triphenylmethylphosphonium (TPMP+) against a concentration gradient, indicating the existence of an electrical potential across the cell membrane (interior negative). Cells exposed to potassium chromate or dichromate (7.7 mumol Cr/l) for 2 h subsequently showed reduced uptake of TPMP+, whereas similar exposure to a trivalent chromium salt (7.7 mumol/l chromic chloride) had similar uptake of TPMP+ as control cells. These preliminary results suggest that a primary effect of chromate on these cells is to reduce the electrical potential across the cell membrane.

Keywords

Cytology and Cytochemistry-Human; Biochemical Studies-Minerals; Biophysics-Membrane Phenomena; Metabolism-Minerals; Toxicology-General; Tissue Culture; In Vitro Studies; Hominidae