US EPA

1237918 

Journal Article 

In Vitro Investigations of Chromiumand Nickel-Hypersensitivity with Culture of Skin and Peripheral Lymphocytes 

Grosfeld, JCM; Penders, AJM; De Grood, R; Verwilghen, L 

1966 

Yes 

Dermatologica
ISSN: 0011-9075 

NIOSH/00129806 

132 

2 

189-198 

English 

5955346 

The growth of skin and lymphocyte cells was studied in patients with chromium (7440473) and nickel (7440020) sensitivity. Skin samples from chromium sensitized and normal subjects were cultured with 0, 0.000001, and 0.0000001 molar concentrations of potassium-bichromate (7778509), all and outgrowth of the cells was determined for 4 days. Peripheral lymphocytes from nickel and chromium sensitized subjects, and from normal subjects, were incubated with 0.000001 to 0.00000001 milliequivalent per milliliter of nickel or chromium antigen. Phytohemagglutinin (PHA) was added to the cultures. Cell mitoses were counted after 72 to 96 hours. No difference in cell outgrowth was found between cell cultures from chromium sensitized and normal subjects, in the presence or absence of potassium-bichromate. Results in peripheral lymphocytes were conclusive: both positive and negative reactions were seen. The authors conclude that the skin culture system is not suitable for demonstrating delayed hypersensitivity in-vitro. The inconsistencies in the lymphocyte tests may be due to a nonspecific effect of the presence of PHA. 

DCN-118365; Chemical properties; Dermatology; In vitro study; Allergic reactions; Skin exposure; Immune system; Blood samples; Hematology; Carcinogenicity