Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
1256791
Reference Type
Journal Article
Title
Natural vanadium-containing Jeju ground water stimulates glucose uptake through the activation of AMP-activated protein kinase in L6 myotubes
Author(s)
Hwang, SL; Chang, HW
Year
2012
Is Peer Reviewed?
1
Journal
Molecular and Cellular Biochemistry
ISSN:
0300-8177
EISSN:
1573-4919
Volume
360
Issue
1-2
Page Numbers
401-409
Language
English
PMID
21948260
DOI
10.1007/s11010-011-1062-4
Web of Science Id
WOS:000297722800043
Abstract
The aim of this study was to elucidate the effects of natural vanadium-containing Jeju ground water on glucose uptake in L6 myotubes and adipogensesis in 3T3 L1 cells. The Jeju ground water samples containing vanadium components were designated as S1 (8.0 ± 0.9 μg/l), S2 (24.0 ± 2.0 μg/l), and S3 (26.0 ± 2.0 μg/l), respectively. To investigate the effects of the Jeju ground water on glucose uptake in L6 myotubes, L6 cells were differentiated in media containing deionized distilled water (DDW group) and the water samples (S1, S2, and S3 groups). After daily changes in cultured media containing the Jeju ground water samples for 1 week, all samples had increased glucose uptake compared to the DDW group and the order of glucose uptake increased in parallel with vanadium content (S3 > S2 > S1). In addition, S3 significantly stimulated the phosphorylation of the Thr-172 residue of the AMP-activated protein kinase-α subunit and the Ser-79 subunit of acetyl-CoA carboxylase compared to the DDW group. The effect of glucose uptake by S3 was reversed by pretreatment with Compound C, an AMPK inhibitor. Interestingly, vanadium pentoxide also increased glucose uptake and activated AMPK activity in a dose-dependent manner. Furthermore, as compared to the DDW treated group, S3 treatment inhibited adipogenesis of 3T3-L1 cells by down regulation of expressions of adipogenic transcription factors. Taken together, these findings suggest that S3 displays beneficial effects in the treatment of diabetes, at least in part through the activation of AMPK activity.
Keywords
Vanadium-containing Jeju ground water; AMP-activated protein kinase; Glucose uptake; Type 2 diabetes; Vanadium pentoxide; Adipogenesis
Tags
IRIS
•
Vanadium Compounds - Problem Formulation
Items Screened
Literature Search: Jan 2010 - Mar 2019
PubMed
WoS
Combined data set
Dataset for title/abstract screening
Supplemental (TIAB)
Supplemental material
Mechanistic
Mixture studies
•
Vanadium Inhalation
Literature Search: Jan 2010 – Mar 2019
PubMed
WoS
Combined Dataset
Dataset for title/abstract screening
Supplemental (TIAB)
Supplemental material
Mechanistic (nongenotoxic)
Non-inhalation route
•
Vanadium Pentoxide (Draft, 2011)
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity