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Citation
Tags
HERO ID
1352575
Reference Type
Journal Article
Title
Glutathione efflux from cultured astrocytes
Author(s)
Sagara, JI; Makino, N; Bannai, S
Year
1996
Is Peer Reviewed?
Yes
Journal
Journal of Neurochemistry
ISSN:
0022-3042
EISSN:
1471-4159
Volume
66
Issue
5
Page Numbers
1876-1881
Language
English
PMID
8780013
Web of Science Id
WOS:A1996UE77600012
Abstract
The characteristics and kinetics of GSH efflux from the monolayer culture of rat astrocytes were investigated. GSH efflux was dependent on temperature, with a Q10 value of 2.0 between 37 and 25 degrees C. The GSH efflux rate showed a hyperbolic dependency on the intracellular GSH concentration. The data were fitted well to the Michaelis-Menten model, giving the following kinetic parameter values: Km = 127 nmol/mg of protein; Vmax = 0.39 nmol/min/mg of protein. p-Chloromercuribenzenesulfonic acid, a thiol-reactive agent impermeable to the cell membrane, lowered the GSH efflux rate by 25% without affecting the intracellular GSH content. These results suggest that a carrier is involved in the efflux of GSH. The GSH content of cultured astrocytes showed a marked increase when the cells were exposed to insults, such as sodium arsenite, cadmium chloride, and glucose/glucose oxidase that lead to the generation of hydrogen peroxide. The increase in GSH content was attributed to the induction of the cystine transport activity by the agents. Although the intracellular GSH concentration and GSH efflux were increased, the kinetics of GSH efflux were not affected by those agents that imposed the oxidative stress. Because the Km value is very large, it is suggested that astrocytes release GSH depending on their GSH concentration in a wide range.
Keywords
glutathione; cystine; transport; astrocytes; oxidative stress
Tags
IRIS
•
Arsenic Hazard ID
1. Initial Lit Search
PubMed
WOS
4. Considered through Oct 2015
6. Cluster Filter through Oct 2015
iAs MOA Literature Categorization
Cytotoxicity and Regenerative Proliferation
Epigenetic Mechanisms
Oxidative Stress
•
Arsenic (Inorganic)
1. Literature
PubMed
Web of Science
4. Adverse Outcome Pathways/Networks Screening
Relevant
•
Arsenic MOA
4. Adverse Outcome Pathways
Oxidative stress related effects (includes non-specific SH reactions)
5. Health Effect
Nervous System Effects
1. MOA Literature Screening
MOA Cluster
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