Health & Environmental Research Online (HERO)


Print Feedback Export to File
1353167 
Journal Article 
Effect of mobile phase composition and pH on thin Layer chromatographic behaviour of amino acids 
Sharma, SD; Sharma, H; Sharma, SC 
2001 
Yes 
Journal of the Indian Chemical Society
ISSN: 0019-4522 
78 
8 
419-421 
English 
WOS:000171350500013 
The chromatographic behaviour of twentyfour amino acids has
been studied on plain silica gel-G layers in one-component, two-component (butanol-acetic acid)
and three-component (acetone-benzene-acetic acid) mobile phases in varying ratios. The effects of
pH and composition of the mobile phase and also of the mole fraction of each component on R-F
values are discussed. The mechanism of migration is explained in terms of adsorption on silica
gel-G and the polarity of the mobile phase.