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1373377 
Journal Article 
Adenosine transporters in chromaffin cells. Quantification by dipyridamol monoacetate 
Torres, M; Molina, P; Miras-Portugal, MT 
1986 
Yes 
FEBS Letters
ISSN: 0014-5793
EISSN: 1873-3468 
201 
1 
124-128 
English 
3709802 
Chromaffin cells from bovine adrenal medulla are a useful model to approach adenosine transport and metabolism in neural cells. Dipyridamol has been shown to be an adenosine transport inhibitor with high affinity. To quantify the adenosine transporters a labelled dipyridamol analogue, [14C]dipyridamol acetate, was synthesized. This compound had a Ki = 5.3 +/- 0.43 nM according to the Dixon method, and 4.58 +/- 0.46 nM when the receptor number molarity was taken into account showing, like dipyridamol, a non-competitive mechanism. The high-affinity receptors present in chromaffin cells showed a Kd = 6.8 +/- 0.8 nM and the receptor number was 630 000 +/- 40 000 per cell.