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1399855 
Journal Article 
Chloroperoxidase: Use of a hydrogen peroxide-stat for controlling reactions and improving enzyme performance 
Vandeurzen, MPJ; Seelbach, K; Vanrantwijk, F; Kragl, U; Sheldon, RA 
1997 
Yes 
Biocatalysis and Biotransformation
ISSN: 1024-2422 
15 
1 
1-16 
WOS:A1997WW41000001 
The use of a hydrogen peroxide-stat for controlling the
hydrogen peroxide concentration has been demonstrated for the chloroperoxidase catalyzed
oxidation of indole. It has been shown that in tert-butyl alcohol/water mixtures the oxidation
can be effectively controlled by a hydrogen peroxide-stat. In this medium chloroperoxidase was
stabilized towards oxidative destruction as long as indole was present in the reaction mixture
but inactivation occurred in the absence of a reductant. In contrast with the results with tert-
butyl alcohol/water mixtures chloroperoxidase was inactivated in water by hydrogen peroxide even
when indole was present in the reaction mixture and the oxidant concentration was as low as 30 mu
M. 
chloroperoxidase; hydrogen peroxide; stabilization; indole; deactivation pathway 
IRIS
• tert-Butanol
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