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1412539 
Journal Article 
Effects of simulated nuclear fuel particles on the histopathology and CYP enzymes in the rat lung and liver 
Pasanen, M; Lang, S; Kojo, A; Kosma, VM 
1995 
Yes 
Environmental Research
ISSN: 0013-9351
EISSN: 1096-0953 
BIOSIS/96/27112 
70 
2 
126-133 
English 
8674481 
We studied both short-term (3 and 30 days) and long-term (3-24 months) effects of simulated nuclear fuel particles (neutron-activated UO2) on the rat lung and liver histopathology and cytochrome P450 (CYP) activities. In the short-term study, after a single intratracheal instillation with neutron-activated particles (administered activity 36 kBq), the lung histology revealed inflammation and a decrease in several lung testosterone hydroxylation levels. Liver exhibited normal histology but hepatic testosterone 7alpha-hydroxylase (T7alphaOH) was decreased by 30% at 3 days treatment with neutron-activated particles (9.3 kBq). At 30 days after treatment, hepatic T7alphaOH and testosterone 15alpha-hydroxylase activities were enhanced by 70 and 40%, respectively. At the long-term follow-up, benign and malignant lung tumors were observed but in the livers only slightly increased inflammation was found. At the 1.5-year follow-up (cumulated lung dose 0.4-0.66 Gy, 131 and 182 kBq), decreases in lung testosterone 6beta-hydroxylase (60%) and testosterone 6alpha-hydroxylase (30%) activities were found. In contrast to lungs, hepatic testosterone 16alpha-hydroxylase activity decreased by 60-75% with both nonactivated and neutron-activated particles. These findings indicate that when lung is exposed to nonactivated UO2 or beta-emitting UO2 particles they have differential effects on CYP enzymes in both the primary target organ (lung) and secondary tissue (liver). 
Microscopy Techniques-Histology and Histochemistry; Radiation-Radiation Effects and Protective Measures; Biochemical Studies-Proteins; Enzymes-Physiological Studies; Pathology; Digestive System-Pathology; Respiratory System-Pathology; Toxicology-Environmental and Industrial Toxicology; Neoplasms and Neoplastic Agents-Carcinogens and Carcinogenesis; Public Health: Environmental Health-Air; Public Health: Environmental Health-Radiation Health; Muridae