US EPA

1434822 

Journal Article 

INCREASED FECUNDITY OF THE CLADOCERAN MOINODAPHNIA-MACLEAYI ON A DIET SUPPLEMENTED WITH A GREEN-ALGA, AND ITS USE IN URANIUM TOXICITY TESTS 

Hyne, RV; Padovan, A; Parry, DL; Renaud, SM 

1993 

Yes 

Australian Journal of Marine and Freshwater Research
ISSN: 0067-1940 

44 

3 

389-399 

WOS:A1993LW86600002 

The effects of two synthetic diets and four algal diets on
the survival and fecundity of Moinodaphnia macleayi for use in life-cycle toxicity tests were
determined. The synthetic foods were a suspension of blended cichlid fish pellets with powdered
alfalfa, either prepared fresh or fermented in natural water for 3 days. The algae, isolated from
the habitat of M. macleayi in the Alligator Rivers Region, were Selenastrum sp., Ankistrodesmus
(falcatus?), Scenedesmus quadricauda and a Chlamydomonas sp. Each alga was tested alone or as a
supplement to the fermented synthetic food. The diet that supported the greatest number of young
and the highest survival rate over a 5-day period was a combination of the fermented synthetic
food and Chlamydomonas sp. Analysis of the fatty acid composition of the lipid fraction of the
four algal species showed that Chlamydomonas sp., Selenastrum sp. and Scenedesmus quadricauda had
significant concentrations of the highly unsaturated fatty acids 18:2(n-6), 18:3(n-6) and 18:3
(n-3). In terms of size and dry weight, Chlamydomonas sp. was the smallest of the algal cells
studied. The toxicity of uranium to M. macleayi survival, determined under standard conditions,
was a lowest-observed-effect concentration (LOEC) of 200 mug L-1 after 48 h and 25 mug L-1 after
5 days. 

MICROALGAE; FATTY ACID; NUTRITION