US EPA

1579853 

Journal Article 

Glutathione depletion in the yeast Saccharomyces cerevisiae 

Fortuniak, A; Zadzinski, R; Bilinski, T; Bartosz, G 

1996 

Yes 

Biochemistry and Molecular Biology International
ISSN: 1039-9712 

BIOSIS/96/21737 

38 

5 

901-910 

English 

9132159 

WOS:A1996UK16500005 

BIOSIS COPYRIGHT: BIOL ABS. The effect of chosen compounds on the total glutathione (GSH) level in stationary cultures of S. cerevisiae was compared. 1-Chloro-2,4-dinitrobenzene, 1-fluoro-2,4-dinitrobenzene, maleimide. iodacetamide and allyl alcohol (1 mM), and menadione (0.5 mM) caused an almost complete GSH depletion during several minutes. Bromobenzoic acid and chloramine T (1 mM), and daunomycin (60 muM) induced a slower GSH decrease, down to 3070% after 60 min. Paraquat (1 mM), CUSO4 (0.5 mM) and cadmium acetate (1 mM) decreased glutathione level down to ca 70%. Diamide (0.5 mM), phenazine methosulphate, phenylhydrazine, acetylphenylhydrazine and H2O2 (1 mM), and t-butyl hydroperoxide (2 mM) did not affect total GSH during 60-min exposure. There was no clear-cut dependence between the ability of various chemicals to deplete cellular GSH and their increased toxicity to a glutathione-poor mutant. 

Biochemical Studies-General; Biochemical Studies-Proteins; Biochemical Studies-Minerals; Toxicology-General; Plant Physiology; Ascomycetes