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HERO ID
190440
Reference Type
Journal Article
Title
Particulate matter inhibits DNA repair and enhances mutagenesis
Author(s)
Mehta, M; Chen, LC; Gordon, T; Rom, W; Tang, MS
Year
2008
Is Peer Reviewed?
1
Journal
Mutation Research: Genetic Toxicology and Environmental Mutagenesis
ISSN:
1383-5718
EISSN:
1879-3592
Volume
657
Issue
2
Page Numbers
116-121
Language
English
PMID
18804180
DOI
10.1016/j.mrgentox.2008.08.015
Web of Science Id
WOS:000261851000005
Abstract
Exposure to ambient air pollution has been associated with adverse health effects including lung cancer. A recent epidemiology study has established that each 10 microg/m3 elevation in long-term exposure to average PM2.5 ambient concentration was associated with approximately 8% of lung cancer mortality. The underlying mechanisms of how PM contributes to lung carcinogenesis, however, remain to be elucidated. We have recently found that transition metals such as nickel and chromium and oxidative stress induced lipid peroxidation metabolites such as aldehydes can greatly inhibit nucleotide excision repair (NER) and enhance carcinogen-induced mutations. Because PM is rich in metal and aldehyde content and can induce oxidative stress, we tested the effect of PM on DNA repair capacity in cultured human lung cells using in vitro DNA repair synthesis and host cell reactivation assays. We found that PM greatly inhibits NER for ultraviolet (UV) light and benzo(a)pyrene diol epoxide (BPDE) induced DNA damage in human lung cells. We further demonstrated that PM exposure can significantly increase both spontaneous and UV-induced mutagenesis. These results together suggest that the carcinogenicity of PM may act through its combined effect on suppression of DNA repair and enhancement of DNA replication errors.
Keywords
Particulate matter; Nucleotide excision repair (NER); Mutagenesis; Host cell reactivation (HCR)
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