US EPA

2202261 

Journal Article 

Determination of toxaphene by basic alcoholic hydrolysis and florisil separation 

Gomes, ED 

1977 

Yes 

Bulletin of Environmental Contamination and Toxicology
ISSN: 0007-4861
EISSN: 1432-0800 

17 

4 

456-462; 1977 

English 

861399 

10.1007/BF01685938 

WOS:A1977DE65000011 

A dehydrohalogenation method for the determination of toxaphene in the presence of other organochlorine pesticides is described. A toxaphene standard was prepared and refluxed with 50% methanolic potassium hydroxide (w/v). The solution was then shaken with water and petroleum ether and separated, the separation procedure being repeated three times followed three additions of water. The petroleum ether layer was evaporated and pipetted onto column containing 60/100 mesh pesticide residue grade florisil. The column was eluted with petroleum ether in two fractions, which were analyzed by electron capture gas chromatography. The relative retention time of the major peak of activity isolated after elution of the toxaphene standard on the florisil column was 1.01 using a 10% DC-200 chromatographic column. On an OV17210 column, the retention time was 1.32. The peak height of the spiked peak on the gas chromatograph was linear with concentration. The spiked peak was specific for toxaphene with little or no interference from pesticides such as chlordane, Strobane, and PCBs, which usually interfere with the determination of toxaphene.