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2289706 
Journal Article 
Molecular and bioenergetic differences between cells with African versus European inherited mitochondrial DNA haplogroups: Implications for population susceptibility to diseases 
Kenney, MC; Chwa, M; Atilano, SR; Falatoonzadeh, P; Ramirez, C; Malik, D; Tarek, M; del Carpio, JC; Nesburn, AB; Boyer, DS; Kuppermann, BD; Vawter, MP; Jazwinski, SM; Miceli, MV; Wallace, DC; Udar, N 
2014 
1 
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
ISSN: 0925-4439
EISSN: 1879-260X 
1842 
2 
208-219 
English 
24200652 
WOS:000331414900010 
The geographic origins of populations can be identified by their maternally inherited mitochondrial DNA (mtDNA) haplogroups. This study compared human cybrids (cytoplasmic hybrids), which are cell lines with identical nuclei but mitochondria from different individuals with mtDNA from either the H haplogroup or L haplogroup backgrounds. The most common European haplogroup is H while individuals of maternal African origin are of the L haplogroup. Despite lower mtDNA copy numbers, L cybrids had higher expression levels for nine mtDNA-encoded respiratory complex genes, decreased ATP (adenosine triphosphate) turnover rates and lower levels of reactive oxygen species production, parameters which are consistent with more efficient oxidative phosphorylation. Surprisingly, GeneChip arrays showed that the Land H cybrids had major differences in expression of genes of the canonical complement system (5 genes), dermatan/chondroitin sulfate biosynthesis (5 genes) and CCR3 (chemokine, CC motif, receptor 3) signaling (9 genes). Quantitative nuclear gene expression studies confirmed that L cybrids had (a) lower expression levels of complement pathway and innate immunity genes and (b) increased levels of inflammation-related signaling genes, which are critical in human diseases. Our data support the hypothesis that mtDNA haplogroups representing populations from different geographic origins may play a role in differential susceptibilities to diseases. (C) 2013 Elsevier B.V. All rights reserved. 
Mitochondrion; Complement activation; Innate immunity; Haplogroup; Cybrid; Retina 
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