US EPA

2799448 

Technical Report 

Pesticide/fertilizer mixture II: reproduction and fertility assessment in Swiss CD-1 mice when administered via drinking water: final report 

Gulati, DK; Grimes, L; Barnes, LH; Chapin, RE; Heindel, J 

1992 

DART/TER/93001595 

NTIS 

NTIS/PB92-140730 

108 pp 

English 

Pesticide/Fertilizer Mixture II (PFM II) is composed of six pesticides (aldicarb, atrazine, dibromochloropropane, 1,2-dichloropropane, ethylene dibromide, and simazine) and 1 fertilizer (ammonium nitrate) and represents a real-life scenario of groundwater contamination in California. PFM II in water was tested for its effects on fertility and reproduction in Swiss CD-1 mice according to the Continuous Breeding Protocol. It was administered via drinking water. Based on an EPA survey, 0, 1, 10, and 100X (1X being the median concentration found in groundwater) were chosen to investigate the effects on fertility and reproduction. Male and female mice (F0) were continuously exposed for a 7-day precohabitation and a 98-day cohabitation period (Task 2). Male and female body weights in Task 2 were within 10% of control values. Water consumption was similar in F0 control and treated animals. Fertility and reproduction in F0 animals were not effected by PFM II. The F1 pups from the final litter in the control, 10 and 100X groups were weaned for second generation studies. Kidney/adrenal weights were approximately 10% higher than controls in F1 10X-treated males and females and 100X-treated females. Absolute seminal vesicle weight as well as seminal vesicle-to-body weight ratio significantly decreased (ca. 11%) in the 100X dose group. PFM II was not a reproductive toxicant in Swiss CD-1 mice at the dose levels tested.