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Citation
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HERO ID
2849879
Reference Type
Journal Article
Title
Extraction and purification of beta-amylase from stems of Abrus precatorius by three phase partitioning
Author(s)
Sagu, ST; Nso, EJ; Homann, T; Kapseu, C; Rawel, HM
Year
2015
Is Peer Reviewed?
Yes
Journal
Food Chemistry
ISSN:
0308-8146
EISSN:
1873-7072
Volume
183
Page Numbers
144-153
Language
English
PMID
25863622
DOI
10.1016/j.foodchem.2015.03.028
Abstract
The stems of Abrus precatorius were used to extract a beta-amylase enriched fraction. A three phase partitioning method and a Doehlert design with 3 variables (ratio of crude extract/t-butanol, the ammonium sulphate saturation and pH) were used. The data was fitted in a second-order polynomial model and the parameters were optimized to enrich beta-amylase. Experimental responses for the modulation were recovery of activity and the purification factor. The optimal conditions were: a ratio of crude extract/t-butanol of 0.87 (v/v), saturation in ammonium sulphate of 49.46% (w/v) and a pH of 5.2. An activity recovery of 156.2% and a purification factor of 10.17 were found. The enriched enzyme was identified as a beta-amylase and its molecular weight was 60.1kDa. Km and Vmax values were 79.37mg/ml and 5.13U/ml, respectively and the highest activity was registered at a temperature of 70°C and a pH between 6 and 6.5. A significant stabilization of the beta-amylase was observed up to 65°C.
Keywords
Abrus precatorius; Beta-amylase; Doehlert design; Purification; Three phase partitioning
Tags
IRIS
•
tert-Butanol
Considered Studies
Electronic Search
Excluded/ Not on Topic
Methodology/Solvent
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