US EPA

3036161 

Journal Article 

Use of coconut water and fertilizer for in vitro proliferation and plantlet production of Dendrobium 'Gradita 31' 

Winarto, B; da Silva, JAT 

2015 

Yes 

In Vitro Cellular and Developmental Biology - Plant
ISSN: 1054-5476
EISSN: 1475-2689 

51 

3 

303-314 

English 

10.1007/s11627-015-9683-z 

WOS:000356457200009 

http://://WOS:000356457200009
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To improve the micropropagation of Dendrobium 'Gradita 31', coconut water (CW) and fertilizer media were used to enhance growth, proliferation, and germination of protocorm-like bodies (PLBs). PLBs formed when shoots approximately 0.4 cm long were cultured on semi-solid half-strength Murashige and Skoog (MS) basal medium containing 1 mg L-1 thidiazuron (TDZ) and 0.5 mg L-1 N-6-benzyladenine (BA). After four rounds of 15-d subcultures, PLBs were initially proliferated in liquid half-strength MS medium supplemented with 0.3 mg L-1 TDZ and 0.1 mg L-1 alpha-naphthalene acetic acid (NAA) and subcultured monthly into fresh medium. When 15% (v/v) CW was added to half-strength MS medium, optimal growth and proliferation of PLBs resulted with the following principal findings: a maximum of 2.86 g total fresh weight (FW) of PLBs formed from 1 g of PLBs, an average of 0.46 g FW of PLBs added per subculture period, a total of 157.1 PLBs formed, an average of 25.3 PLBs added per subculture period, and a low percentage of PLB browning (20.7%). Half-strength MS medium containing 15% (v/v) CW with Rosasol(A (R)) medium (liquid fertilizer; 1.5 g L-1 18 N:18P:18 K + 1.5 g L-1 25 N:10P:10 K + TE) resulted in a similar organogenic outcome with 25.5% PLB browning. PLBs germinated easily and rooted on Rosasol-supplemented plant growth regulator-free medium. In total, 180 Dendrobium 'Gradita 31' plantlets from all treatments were successfully acclimatized with 100% survival on a Cycas rumphii bulk substrate and grew well after repotting in a mixture of wood charcoal and C. rumphii bulk (1:1, v/v). 

Organic additive; Rosasol (R) medium; Proliferation; Protocorm-like body