US EPA

3697367 

Journal Article 

Single gas chromatography method with nitrogen phosphorus detector for urinary cotinine determination in passive and active smokers 

Malafatti, L; Maia, PP; Goncalves Martins, MC; Pereira Bastos de Siqueira, ME; Martins, I 

2010 

Yes 

B J P S Brazilian Journal of Pharmaceutical Sciences
ISSN: 1984-8250 

46 

4 

769-776 

10.1590/S1984-82502010000400019 

WOS:000208891500019 

Nicotine is a major addictive compound in cigarettes and is rapidly and extensively metabolized to several metabolites in humans, including urinary cotinine, considered a biomarker due to its high concentration compared to other metabolites. The aim of this study was to develop a single method for determination of urinary cotinine, in active and passive smokers, by gas chromatography with a nitrogen phosphorus detector (GC-NPD). Urine (5.0 mL) was extracted with 1.0 mL of sodium hydroxide 5 mol L-1, 5.0 mL of chloroform, and lidocaine used as the internal standard. Injection volume was 1 mu L in GC-NPD. Limit of quantification was 10 ng mL(-1). Linearity was evaluated in the ranges 10-1000 ng mL(-1) and 500-6000 ng mL(-1), with determination coefficients of 0.9986 and 0.9952, respectively. Intra-and inter-assay standard relative deviations were lower than 14.2 %, while inaccuracy (bias) was less than + 11.9%. The efficiency of extraction was greater than 88.5%. Ruggedness was verified, according to Youden's test. Means of cotinine concentrations observed were 2,980 ng mL(-1) for active smokers and 132 ng mL(-1), for passive smokers. The results revealed that satisfactory chromatographic separation between the analyte and interferents was obtained with a ZB-1 column. This method is reliable, precise, linear and presented ruggedness in the range evaluated. The results suggest that it can be applied in routine analysis for passive and active smokers, since it is able to quantify a wide range of cotinine concentrations in urine. 

Gas chromatography/with nitrogen phosphorus detector; Urinary cotinine/determination; Urine/toxicological analysis; Smokers/Toxicology