US EPA

3718389 

Journal Article 

Optimization of a single phase method for lipid extraction from milk 

Liu, Z; Rochfort, S; Cocks, BG 

2016 

Yes 

Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778 

1458 

145-149 

English 

27372415 

10.1016/j.chroma.2016.06.055 

For LC-MS-based lipidomic analysis of milk, total lipid extraction from raw milk is generally conducted with Folch or Bligh and Dyer methods; both methods are based on two-phase partition of lipids, and thus time-consuming. In this work, three solvent systems for one-phase extraction of milk lipids were compared with the standard Folch method. Two of the solvent systems (butanol/methanol, 3:1 and 1:1) previously tested for lipid extraction from plasma were found to provide adequate extraction for polar lipids, but incomplete extraction for triglycerides, especially highly lipophilic species. By contrast, our newly designed solvent mix composed of butanol, methanol and chloroform (at a 3:5:4 ratio) provided similar extraction efficiency for triglycerides and higher yield for some of the phospholipids, as compared to the Folch method. This new one-phase extraction method is very simple yet comprehensive and thus suitable for high throughput lipid analysis of milk samples.