US EPA

3814505 

Journal Article 

Effects of metamizol and magnesium sulfate on enzyme activity of glucose 6-phosphate dehydrogenase from human erythrocyte in vitro and rat erythrocyte in vivo 

Ciftçi, M; Ozmen, I; Büyükokuroğlu, ME; Pençe, S; Küfrevioğlu, OI 

2001 

Yes 

Clinical Biochemistry
ISSN: 0009-9120
EISSN: 1873-2933 

34 

4 

297-302 

English 

11440730 

10.1016/s0009-9120(01)00207-7 

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034964656&doi=10.1016%2fS0009-9120%2801%2900207-7&partnerID=40&md5=91282145728548bc42939cd886d8ce55
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OBJECTIVE: Effects of metamizol and magnesium sulfate on erythrocyte glucose 6-phosphate dehydrogenase enzyme activity were investigated in in vitro and in vivo conditions.

METHODS: For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme activity was determined according to the Beutler method by using a spectrophotometer at 340 nm.

RESULTS: The results of in vitro study showed that their mean K(i) values were 6.35 x 10(-3) M for metamizol and 1.32 x 10(-2) M for magnesium sulfate and their inhibition types were uncompetitive. I(50) value was 17 mM for metamizol and 50 mM for magnesium sulfate in in vitro study. In the case of in vivo studies, 200 mg/kg metamizol inhibited the enzyme activity by 40% during the first 1.5 h (p < 0.05), and 225 mg/kg magnesium sulfate significantly inhibited the enzyme activity throughout 24 h (p < 0.01).

CONCLUSION: The results of this study suggested that metamizol and magnesium sulfate have significant inhibition effect on the activity of glucose 6-phosphate dehydrogenase enzyme in both in vivo and in vitro. 

Glucose 6-phosphate dehydrogenase; Magnesium sulfate; Metamizol