US EPA

3862292 

Journal Article 

Development of a topical mupirocin spray for antibacterial and wound-healing applications 

Sritharadol, R; Nakpheng, T; Wan Sia Heng, P; Srichana, T 

2017 

Yes 

Drug Development and Industrial Pharmacy
ISSN: 0363-9045
EISSN: 1520-5762 

43 

10 

1-14 

English 

28581830 

10.1080/03639045.2017.1339077 

WOS:000407060900015 

OBJECTIVE: The aim of this study was to develop mupirocin topical spray using Eudragit E100 as a film-forming agent for the treatment of bacterial skin infections as well as to promote wound healing.

MATERIALS AND METHODS: Twenty-seven of mupirocin formulations were formulated containing Eudragit E100 and other excipients. Mupirocin spray was prepared by aerosol crimping and filling machine using HFA-134a as a propellant. The formulations were evaluated for their stability and physicochemical properties. The factorial study was applied to evaluate the effects of glycerol and PEG400 on mupirocin-loaded Eudragit E100 films. The optimized formulation was assessed of drug release, antibacterial activities and in vitro cell line studies in comparison to the ointment formulation.

RESULTS AND DISCUSSION: Mupirocin sprays were formulated and optimized to obtain the formulation with excellent physicochemical and mechanical properties of the dressing film. The formulation had an excellent stability up to a year with more than 80% of mupirocin content. Mupirocin was released from the film up to 90% within 2 h. The formulation had a potent antibacterial effect against S. aureus and S. epidermidis. The formulation was safe to use as a topical formulation that had no toxicity to keratinocytes, fibroblasts and monocytes. The formulation also had an antiendotoxin effect without stimulating the production of NO and inflammatory cytokines (IL-1β and TNF-α).

CONCLUSIONS: Mupirocin topical spray was successful developed as a topical formulation and can be used instead of the ointment formulation. Animal experiments are warranted to further emphasize the safe use in the human skin. 

Administration, Topical; Animals; Anti-Bacterial Agents/administration & dosage/chemistry/pharmacology; Chemistry, Pharmaceutical; Hydrocarbons, Fluorinated/chemistry; Mupirocin/administration & dosage/chemistry/pharmacology; Staphylococcus aureus/chemistry/drug effects; Staphylococcus epidermidis/chemistry/drug effects; Tumor Necrosis Factor-alpha/chemistry/pharmacology; Wound Healing/drug effects/physiology; Eudragit E100; Mupirocin; anti-infectives; biomaterials; topical spray; wound healing