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4160508 
Journal Article 
The sensitivity and specificity of the mTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines 
Malich, G; Markovic, B; Winder, C 
1997 
1 
Toxicology
ISSN: 0300-483X
EISSN: 1879-3185 
BIOSIS/98/09862 
124 
3 
179-192 
English 
BIOSIS COPYRIGHT: BIOL ABS. A number of studies reported that the MTS in vitro cytotoxicity assay is a convenient method for assessing cell viability. The main features found with this assay are its ease of use, accuracy and rapid indication of toxicity. It might well be a useful tool in human health risk assessment if it can be shown that this assay also has an acceptable sensitivity and specificity. This is of interest particularly when exposure to unknown chemical substances requires the rapid detection and evaluation of toxic effects. In this study, the cytotoxicity of 20 chemicals selected from the MEIC priority list was determined with the MTS assay. Since it could be shown that interactions between detection reagents with published toxicity data of the same chemicals established with in vitro and in vivo toxicological test systems. Direct comparisons of the data showed a generally lower sensitivity of the MTS assay, which is influenced by when the MTS assay was compared with in vivo studies 
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