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4245323 
Journal Article 
Ozonation control and effects of ozone on water quality in recirculating aquaculture systems 
Spiliotopoulou, A; Rojas-Tirado, P; Chhetri, RK; Kaarsholm, KMS; Martin, R; Pedersen, PB; Pedersen, LF; Andersen, HR 
2018 
1 
Water Research
ISSN: 0043-1354
EISSN: 1879-2448 
Elsevier 
England 
133 
289-298 
English 
29407710 
WOS:000426331900029 
To address the undesired effect of chemotherapeutants in aquaculture, ozone has been suggested as an alternative to improve water quality. To ensure safe and robust treatment, it is vital to define the ozone demand and ozone kinetics of the specific water matrix to avoid ozone overdose. Different ozone dosages were applied to water in freshwater recirculating aquaculture systems (RAS). Experiments were performed to investigate ozone kinetics and demand, and to evaluate the effects on the water quality, particularly in relation to fluorescent organic matter. This study aimed at predicting a suitable ozone dosage for water treatment based on daily ozone demand via laboratory studies. These ozone dosages will be eventually applied and maintained at these levels in pilot-scale RAS to verify predictions. Selected water quality parameters were measured, including natural fluorescence and organic compound concentration changes during ozonation. Ozone reactions were described by first order kinetics. Organic matter, assessed as chemical oxygen demand and fluorescence, decreased by 25% (low O3), 30% (middle O3) and 53% (high O3), while water transmittance improved by 15% over an 8-day period. No fish mortality was observed. Overall, this study confirms that ozone can improve RAS water quality, provides a better understanding of the ozone decay mechanisms that can be used to define further safe ozone treatment margins, and that fluorescence could be used as a monitoring tool to control ozone. This study might be used as a tool to design ozone systems for full-scale RAS by analysing water sample from the specific RAS in the laboratory. 
Fluorescence; Laboratory study; Ozone; Pilot-scale; RAS; Water quality 
Water research