Health & Environmental Research Online (HERO)


Print Feedback Export to File
4245648 
Journal Article 
Structural characterization of gangliosides and glycolipids via ultraviolet photodissociation mass spectrometry 
O'Brien, JP; Brodbelt, JS 
2013 
Yes 
Analytical Chemistry
ISSN: 0003-2700
EISSN: 1520-6882 
85 
21 
10399-10407 
English 
24083420 
WOS:000326711400061 
Ultraviolet photodissociation (UVPD) mass spectrometry was used to characterize the structures of amphiphilic glycosphingolipids and gangliosides in comparison to collision induced dissociation (CID) and higher energy collision dissociation (HCD) in a high performance Orbitrap mass spectrometer. UVPD produced the widest array of fragment ions diagnostic for both the ceramide base and oligosaccharide moieties. CID and HCD generated mainly glycosidic B/Y and C/Z cleavages of the oligosaccharides moieties and very few informative fragments related to the hydrophobic ceramide base. Several unique cleavages at the sphingoid base and the fatty acid chain occurred upon UVPD, as well as a wider variety of cross ring cleavages (A/X ions), thus affording differentiation of isobaric gangliosides. An LC-UVPD-MS strategy allowed the elucidation of 27 gangliosides among five different classes.