US EPA

4257516 

Journal Article 

Comparison of Intramammary Ozone Administration Doses in Dairy Cows with Clinical Mastitis 

Enginler, SO; Sabuncu, A; Kahraman, BB; Kocak, O; Yildar, E; Guzel, O 

2015 

Yes 

Acta Scientiae Veterinariae
ISSN: 1678-0345
EISSN: 1679-9216 

43 

WOS:000356393000006 

Background: Mastitis is an economically important disease leading cost associated problems. The most accurate relationship between intramammary infection and somatic cell count (SCC) can be evaluated at quarter level when SCC exceeds 100.000 cells/mL. In subclinical mastitis, pathogens do not cause enough disruption in the alveolar tissue to be seen in the milk but SCC increase indicates the infected milk. Ozone (O-3) is an unstable polymerized oxygen created by the passage of air or oxygen over high energy electrodes within an ozone generator system or by ultraviolet light. The aim of this study was to compare the treatment efficacy of ozone (O-3) insufflation daily via latex free syringe in different doses (30-60-70 mu g/mL) and high dose O-3 insufflation (70 mu g/mL) with combination of antibiotic and only antibiotic treatment in dairy cows with clinical mastitis.



Materials, Methods & Results: A total of 32 lactating cows with clinical signs of mastitis whom had got 79 infected mammary quarters were used for the study. Mastitis was diagnosed by typical clinical symptoms of the mammary gland and general clinical symptoms of systemic infection. California mastitis test (CMT) was performed to all quarters and recorded before the treatment. Somatic cell count (SCC) of each milk sample of the infected quarters was recorded before the treatment. Microbiological examination was conducted on aseptically collected milk samples from infected quarters before the treatment. Ozone insufflation was applied daily in different doses (30, 60 and 70 mu g/mL) intramammary via latex free 100 mL syringe every day for one week and only antibiotic was administered intramuscularly to the mastitic cows for 5 days and high dose ozone insufflation (70 mu g/mL) with combination of antibiotic therapy after milking of the animals. On day 8; CMT and SCC were repeated to evaluate the efficacy of the treatments on the milk samples. The statistical analysis of CMT and SCC values between groups was conducted according to Paired Sample t-test and P value of < 0.05 was considered significant.



Discussion: In the current study, CMT scores were found to best decreased in 70 mu g/mL O-3 + AB combination group in post-treatment (1.63 +/- 0.10) scores when compared to pre-treatment (1.21 +/- 0.08) scores and the difference was found statistically significant (P < 0.01) between pre- and post-treatment of CMT scores. In another study, the initial CMT score was reported as 10 +/- 0.97 and found to be decreased to 4.55 +/- 0.83 on the 3rd day after ozone treatment. SCC values were reported as decreased gradually towards to normal range after 3 weeks ozone and antibiotic treatment in a study. SCC results are tended to be decreased in this study in line with their study after one week therapy but in this study SCC values did not reach to normal reference ranges. It may be due to the shorter treatment duration of this study when compared to their study. The most isolated bacteria was detected as E. coli and Staphylococcus aureus (n=12 for each) in the present study, S. aureus was also the most isolated bacteria (n=7) in a previously published study. In conclusion, high doses ozone (60-70%) and high dose ozone (70%) in combination with antibiotic can be effectively used to treat clinical mastitis cases in dairy cattle. The best result was achieved in high dose ozone (70 mu g/mL) with combination of antibiotic therapy in the cases of clinical mastitis in dairy cattle in current study. 

antibiotic; clinical mastitis; intramammary insufflation; ozone therapy