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72190 
Journal Article 
Mutation of human cells by kerosene soot 
Skopek, TR; Liber, HL; Kaden, DA; Hites, RA; Thilly, WG 
1979 
Yes 
Journal of the National Cancer Institute
ISSN: 0027-8874
EISSN: 1460-2105 
63 
2 
309-312 
English 
379398 
The polycyclic aromatic hydrocarbon fraction of kerosene soot induced forward mutation in human diploid lymphoblasts when coincubated with Sprague-Dawley rat liver postmitochondrial supernatant. Two components of the kerosene soot extract, benzo(a)pyrene (BP) and cyclopenta(cd)pyrene (CP), were also tested. BP was not mutagenic at the concentration found in the soot extract, although its was active at higher concentrations. The amount of CP present could account forof the total mutation observed with the soot. Results were compared to data obtained previously in a similar mutation assay in Salmonella typhimurium. The protocol described permits the facile assay of mutation at the hgprt (hypoxanthine guanine phosphoribosyl transferase) locus in human lymphoblasts; such mutation is induced by compounds or complex mixtures requiring mixed-function oxygenase activity for metabolism to genetically active derivatives. (Applicability to carcinogenesis is discussed.) 
• PCBs
     Excluded