US EPA

597864 

Book/Book Chapter 

Exposure to styrene: Comparison of DNA and haemoglobin adducts as biomarkers 

Latriano, L; Wazneh, L; Dong, Z; Lu, SJ; Snyder, C; Jeffrey, AM 

1991 

Oxford University Press 

New York, NY 

NIOSH/00228655 

Human carcinogen exposure. Biomonitoring and risk assessment 

Biomonitoring and Risk Assessment 

305-314 

is a chapter of 1061267 Human carcinogen exposure: Biomonitoring and risk assessment

The use of phosphorus-32 (P32) postlabeling for the identification of styrene-oxide adducts was discussed and compared with the analysis of hemoglobin adducts. DNA isolated from the lungs, livers, and lymphocytes of male Fischer-rats exposed to 1000 parts per million styrene (100425) vapors for 6 hours/day for 5 days were analyzed as well as calf thymus DNA modified in-vitro with styrene-oxide (96093). Mutagenicity assays were also performed with Salmonella-typhimurium (TA-100) exposed to styrene-oxide at doses up to 16 micromoles/plate. The analysis of DNA using P32 postlabeling and the separation and purification of P32 labeled styrene-oxide/DNA adducts were described. Procedures for the isolation of globin from rat erythrocytes and the subsequent gas chromatographic/mass spectrometric analysis for hemoglobin adducts after a reaction with pentafluorophenylisothiocyanate were performed. Seven adducts were separated following P32 postlabeling and chromatography using five solvents. The limit of detection of the P32 assay was 1 adduct/10(4) nucleotides. The sensitivity of the method was enhanced using both the nuclease-P1 and butanol procedures for DNA adduct enrichment. A mutagenic response was seen following exposure of S-typhimurium to styrene-oxide, with DNA adducts detected only following exposure to 20 micromoles/milliliter or more. Identical adduct patterns were seen in styrene as well as air exposed rats after P32 postlabeling of lung, liver, or lymphocyte DNA. The analysis of hemoglobin adducts yielded similar retention times for the styrene-oxide/valine derivatives. 

DCN-230018; Laboratory techniques; Analytical methods; DNA adducts; DNA damage; Organic solvents; Biological monitoring; Chromatographic analysis; Quantitative analysis; Radioassays; In vitro studies; Aromatic hydrocarbons