US EPA

6396373 

Journal Article 

Synthesis of a Colorimetric Fluorescent Probe of Cr3+ and Its Application in Cell Imaging 

Zhang Yingying; Huang Yiwen; Zhao Bing; Wang Liyan; Song Bo 

2019 

1 

Gaodeng Xuexiao Huaxue Xuebao / Chemical Journal of Chinese Universities
ISSN: 0251-0790 

40 

12 

2486-2493 

10.7503/cjcu20190354 

WOS:000504320800009 

Colorimetric fluorescence probe B was synthesized from 2, 3, 3-trimethyl-3H-benzo [e] indole and p-dimethylaminobenzaldehyde using acidic solvent as catalyst and ethanol as solvent. In the mixed system (pH = 7. 4) of V(EtOH) V(HEPES) = 9 1, the color of solution B changed from pale yellow to purple-red after added Cr3+ to probe B solution, indicating that probe B can recognize Cr3+ with naked-eye. Fluorescence emission spectra shows that probe B had good selectivity of Cr3+, high sensitivity and good recognition ability with EDTA. The binding constant(K-a) of probe B on Cr3+ was 0.28 x 102 mol/L and the detection limit was 1. 90x 10(-8) mol/L. The detection limit was lower than the maximum Cr3+ content (9. 60 x 10(-8) mol/L) in drinking water stipulated by WHO. In addition, the concentration of Cr3+ in actual water samples was quantitatively detected by fluorescence emission spectra. Probe B has been applied to the detection of Cr3+ in living cells, which has a good application prospect and practical value.< 

Benzindole; Fluorescence probe; Cr3+; Water sample; Cell