US EPA

6578123 

Journal Article 

Abstract 

Capsicum peppers screening against powdery mildew and study of in vitro media as alternative to preserve the inoculum 

Morales Manzo, II; Fita, A; San Bautista, A; Rodriguez Burruezo, A 

2019 

Yes 

Journal of Biotechnology
ISSN: 0168-1656
EISSN: 1873-4863 

305 

Suppl. 

S67-S68 

English 

10.1016/j.jbiotec.2019.05.237 

WOS:000491118400219 

The relevance of powdery mildew (Leveillula taurica (LEv.) Arn.) as a disease in peppers has increased in the last years, particularly in organic production due to a lower use of chemicals. In this work, a collection of Capsicum accessions was preliminary screened against powdery mildew by spraying plants with conidia, grown in minigreenhouses situated within growth chambers. Plants inoculated were evaluated by scoring affected leaves and percentage of affected area at 4 and 8 weeks after inoculation. Four interesting varieties were identified, two resistant, one tolerant, and one highly susceptible. They will be re-evaluated again in future screenings to confirm these results and integrate them in breeding programs and experimental populations. Furthermore, two sterilizing treatments of infected leaves (via sodium hypochlorite and mercury chloride) and two growth media (Murashige&Skoog with vitamins and Murashige&Skoog with Nitsch vitamins) as a maintenance alternative of infected tissues (leaves) were tested. No significant differences were found between sterilizing treatments, although the selected one should be sodium hypochlorite due to its lower human's health and enviromental hazard. M&S with Nitsch vitamins medium provided a better development of conidiophores and conidia (30% occurrence). 

European Biotechnology Congress 

Valencia, Spain 

April 11-13, 2019