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700534 
Journal Article 
Pharmacokinetic analysis of chloral hydrate and its metabolism in B6C3F1 mice 
Abbas, RR; Seckel, CS; Kidney, JK; Fisher, JW 
1996 
Yes 
Drug Metabolism and Disposition
ISSN: 0090-9556
EISSN: 1521-009X 
24 
12 
1340-1346 
English 
Chloral hydrate (CH) and its metabolites, trichloroacetate (TCA) and dichloroacetate (DCA), have been shown to induce liver tumors in male B6C3F1 mice. The pharmacokinetics of CH and its metabolites play an important role in its toxicity. This study was designed to characterize the kinetics of CH metabolism, and the formation and elimination of TCA, DCA, trichloroethanol (TCOH), and trichloroethanol glucuronide (TCOG) in male B6C3F1 mice. Mice were dosed with 67.8, 678, and 2034 micromol/kg of CH through the tail vein. At selected time points, mice were killed, and blood and liver samples were collected. Samples were assayed by GC for CH, TCOH, TCOG, TCA, and DCA concentrations. After intravenous administration, CH rapidly disappeared from blood with a terminal half-life ranging from 5 to 24 min. Systemic clearance decreased from 36.0 to 7.6 liters/kg-hr with increasing CH dose, demonstrating dose-dependent pharmacokinetics. TCOH, TCOG, TCA, and DCA were detected over the study period. Formation and metabolism of CH metabolites seemed to be dose-dependent. The terminal half-lives of TCOH and TCOG were similar, ranging from 0.2 to 0.7 hr. TCA and DCA were formed rapidly from the metabolism of CH and cleared slowly from systemic circulation. The area under the blood concentration-time curve for DCA was 10-20% of that for TCA. Both TCA and DCA were slowly eliminated from systemic circulation. The concentration-time profile of DCA seemed to be driven by the blood concentration of TCA, suggesting the possibility of DCA formation from TCA metabolism. 
Animals 
Area Under Curve 
Biotransformation 
Chloral Hydrate 
Dichloroacetate 
Glucuronates 
Half-Life 
Hypnotics and Sedatives 
Injections, Intravenous 
Liver 
Male 
Mice 
Mice, Inbred Strains 
Trichloroacetic Acid