Health & Environmental Research Online (HERO)


Print Feedback Export to File
724812 
Technical Report 
Dichloroacetic Acid Metabolism In Vitro: I. Investigation of the Factors Influencing Dichloroacetic Acid Metabolism 
Mahle, DA; Buttler, GW; Lipscomb, JC 
1995 
Govt Reports Announcements & Index (GRA&I), Issue 18, 1997 
NTIS/02997005 
GRA and I 
GRA and I 
TD3: Dichioroacetic acid (DCA) is a major metabolite of trichioroethylene (TRI), a common groundwater contaminant. Dichioroacetic acid has been reported to cause hepatocarcinomas in rodents. We have shown that DCA metabolism is dependent on a cytosolic protein and, therefore, not P-450 dependent. However, the products of DCA metabolism have not been clearly identified. Initial experiments performed with nuclease and protease ruled out binding of DCA as an explanation for the disappearance of DCA from cytosolic incubations. Experiments were then conducted to determine if a specific cofactor dependence for DCA metabolism existed. Mouse liver cytosol was incubated with either nicotinamide or flavin cofactors at a concentration of 0.9 mM or 0.24-5 mM glutathione (GSH) and with 20.50 ug/mI DCA for 3 to 20 minutes at 370C. The incubations were derivatized and analyzed to assess DCA removed from solution. Dichloroacetic acid metabolism increased with increasing concentration of GSH. Mouse liver cytosol was 
A