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737817 
Journal Article 
Binding assay for cholera toxin based on sequestration electrochemistry using lactose labeled with an electroactive compound 
Kuramitz, H; Miyagaki, S; Ueno, E; Hata, N; Taguchi, S; Sugawara, K 
2011 
1 
Analyst
ISSN: 0003-2654
EISSN: 1364-5528 
136 
11 
2373-2378 
English 
21491033 
A simple electrochemical binding assay for cholera toxin (CT) was developed using lactose labeled with daunomycin as an electroactive compound. The labeled lactose (LL) was determined with high sensitivity by adsorptive stripping voltammetry (AdSV). The electrochemical behaviors of LL at glassy carbon (GC), plastic formed carbon (PFC) and carbon nanotubes paste (CNTP) electrode were investigated. The CNTP electrode showed the greatest accumulation capacity for LL. The assay for CT based on the sequestration electrochemistry was demonstrated. The binding event of the LL to CT was detected by the decrease in the electrochemical response of daunomycin as an electroactive label without a separation process to remove the free LL from the one bound with CT before any measurements can be made. The detection limit of the CT assay using the CNTP electrode was 0.5 nM (42 ng mL(-1)). 
Other
• Nanoscale Carbon