Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
759001
Reference Type
Journal Article
Title
The role of iron in Libby amphibole-induced acute lung injury and inflammation
Author(s)
Shannahan, JH; Ghio, AJ; Schladweiler, MC; Mcgee, JK; Richards, JH; Gavett, SH; Kodavanti, UP
Year
2011
Is Peer Reviewed?
Yes
Journal
Inhalation Toxicology
ISSN:
0895-8378
EISSN:
1091-7691
Volume
23
Issue
6
Page Numbers
313-323
Language
English
PMID
21605006
DOI
10.3109/08958378.2011.569587
Web of Science Id
WOS:000290935500001
URL
http:///www.informahealthcare.com
Exit
Abstract
Complexation of host iron (Fe) on the surface of inhaled asbestos fibers has been postulated to cause oxidative stress contributing to in vivo pulmonary injury and inflammation. We examined the role of Fe in Libby amphibole (LA; mean length 4.99 µm ± 4.53 and width 0.28 µm ± 0.19) asbestos-induced inflammogenic effects in vitro and in vivo. LA contained acid-leachable Fe and silicon. In a cell-free media containing FeCl(3), LA bound #17 µg of Fe/mg of fiber and increased reactive oxygen species generation #3.5 fold, which was reduced by deferoxamine (DEF) treatment. In BEAS-2B cells exposure to LA, LA loaded with Fe (FeLA), or LA with DEF did not increase HO-1 or ferritin mRNA expression. LA increased IL-8 expression, which was reduced by Fe loading but increased by DEF. To determine the role of Fe in LA-induced lung injury in vivo, spontaneously hypertensive rats were exposed intratracheally to either saline (300 µL), DEF (1 mg), FeCl(3) (21 µg), LA (0.5 mg), FeLA (0.5 mg), or LA + DEF (0.5 mg). LA caused BALF neutrophils to increase 24 h post-exposure. Loading of Fe on LA but not chelation slightly decreased neutrophilic influx (LA + DEF > LA > FeLA). At 4 h post-exposure, LA-induced lung expression of MIP-2 was reduced in rats exposed to FeLA but increased by LA + DEF (LA + DEF > LA > FeLA). Ferritin mRNA was elevated in rats exposed to FeLA compared to LA. In conclusion, the acute inflammatory response to respirable fibers and particles may be inhibited in the presence of surface-complexed or cellular bioavailable Fe. Cell and tissue Fe-overload conditions may influence the pulmonary injury and inflammation caused by fibers.
Keywords
Libby amphibole; iron homeostasis; inflammation
Tags
IRIS
•
Asbestos
•
Libby Amphibole Asbestos (Draft, 2011)
OPPT REs
•
OPPT_Asbestos, Part I: Chrysotile_F. Human Health
Total – title/abstract screening
On topic
Peer review
Primary source
Other forms of asbestos
•
OPPT_Asbestos, Part I: Chrysotile_Supplemental Search
LitSearch: Sept 2020 (Undated)
PubMed
Toxline
WoS
Legacy Uses
Health Outcomes
Additional Legacy Terms
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity