Calcium-sensing receptor regulates stomatal closure through hydrogen peroxide and nitric oxide in response to extracellular calcium in Arabidopsis | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

846251

Reference Type

Journal Article

Title

Calcium-sensing receptor regulates stomatal closure through hydrogen peroxide and nitric oxide in response to extracellular calcium in Arabidopsis

Author(s)

Wang, WH; Yi, XQ; Han, AD; Liu, TW; Chen, J; Wu, FH; Dong, XJ; He, JX; Pei, ZM; Zheng, HL

Year

2012

Is Peer Reviewed?

Yes

Journal

Journal of Experimental Botany
ISSN: 0022-0957
EISSN: 1460-2431

Volume

Issue

Page Numbers

177-190

Language

English

PMID

21940718

DOI

10.1093/jxb/err259

Web of Science Id

WOS:000298383400014

URL

https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/err259
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Abstract

The Arabidopsis calcium-sensing receptor CAS is a crucial regulator of extracellular calcium-induced stomatal closure. Free cytosolic Ca(2+) (Ca(2+)(i)) increases in response to a high extracellular calcium (Ca(2+)(o)) level through a CAS signalling pathway and finally leads to stomatal closure. Multidisciplinary approaches including histochemical, pharmacological, fluorescent, electrochemical, and molecular biological methods were used to discuss the relationship of hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) signalling in the CAS signalling pathway in guard cells in response to Ca(2+)(o). Here it is shown that Ca(2+)(o) could induce H(2)O(2) and NO production from guard cells but only H(2)O(2) from chloroplasts, leading to stomatal closure. In addition, the CASas mutant, the atrbohD/F double mutant, and the Atnoa1 mutant were all insensitive to Ca(2+)(o)-stimulated stomatal closure, as well as H(2)O(2) and NO elevation in the case of CASas. Furthermore, it was found that the antioxidant system might function as a mediator in Ca(2+)(o) and H(2)O(2) signalling in guard cells. The results suggest a hypothetical model whereby Ca(2+)(o) induces H(2)O(2) and NO accumulation in guard cells through the CAS signalling pathway, which further triggers Ca(2+)(i) transients and finally stomatal closure. The possible cross-talk of Ca(2+)(o) and abscisic acid signalling as well as the antioxidant system are discussed.

Keywords

ABA signalling; antioxidant system; calcium-sensing receptor; extracellular calcium signalling; guard cells; hydrogen peroxide; nitric oxide