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HERO ID
880682
Reference Type
Journal Article
Title
Hericium erinaceus suppresses LPS-induced pro-inflammation gene activation in RAW264.7 macrophages
Author(s)
Kim, YO; Lee, SW; Oh, CH; Rhee, YH
Year
In Press
Is Peer Reviewed?
Yes
Journal
Immunopharmacology and Immunotoxicology
ISSN:
0892-3973
EISSN:
1532-2513
Language
English
PMID
22126451
DOI
10.3109/08923973.2011.633527
Web of Science Id
WOS:000303830400021
Abstract
The aim of this study was to investigate the anti-inflammatory properties of each fraction of Hericium erinaceus (HE). The ethanol extract from HE was partitioned with different solvents in the order of increasing polarity. The treatment with 10-100 μg/mL of each fraction did not reduce RAW 264.7 cell viability except ethyl acetate fraction. Among the various extracts, the chloroform fraction showed the most potent activity against nitric oxide (NO), prostaglandin E(2) (PGE(2)) and reactive oxygen species (ROS). The western blotting and reverse transcriptase polymerase chain reaction (RT-PCR) analyses revealed that chloroform fraction from HE (CHE) significantly reduced the protein level of iNOS and cyclooxygenase-2 (COX-2) or mRNA levels of iNOS in lipopolysaccharide-induced macrophages. Furthermore, CHE inhibited the translocation of nuclear factor (NF)-κB p65 subunit, phsophorylation of I-κB, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK) in a dose-dependent manner. Furthermore, the activation of both activator protein-1 (AP-1) and NF κB in the nucleus were abrogated by CHE with luciferase assay. In conclusion, these results indicate that CHE may provide an anti-inflammatory effect by attenuating the generation of excessive NO, PGE(2), and ROS and by suppressing the expression of pro-inflammatory genes through the inhibition of NF-κB and JNK activity.
Keywords
Hericium erinaceus; RAW 264. 7 macrophages; anti-inflammation effect; nuclear factor kappa B
Tags
•
Chloroform 2018 Update
TITLE AND ABSTRACT REVIEW
Excluded Studies
•
Chloroform Combined (current)
Chloroform (original)
References: 2000-2018
WoS
Chloroform (2018 update)
TITLE AND ABSTRACT REVIEW
Excluded Studies
Chloroform (current)
Literature Search: Jan 2009 - March 2017
Web of Science
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