Impacts of gene bioaugmentation with pJP4-harboring bacteria of 2,4-D-contaminated soil slurry on the indigenous microbial community
Inoue, D; Yamazaki, Y; Tsutsui, H; Sei, K; Soda, S; Fujita, M; Ike, M
HERO ID
1000131
Reference Type
Journal Article
Year
2012
Language
English
PMID
| HERO ID | 1000131 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Impacts of gene bioaugmentation with pJP4-harboring bacteria of 2,4-D-contaminated soil slurry on the indigenous microbial community |
| Authors | Inoue, D; Yamazaki, Y; Tsutsui, H; Sei, K; Soda, S; Fujita, M; Ike, M |
| Journal | Biodegradation |
| Volume | 23 |
| Issue | 2 |
| Page Numbers | 263-276 |
| Abstract | Gene bioaugmentation is a bioremediation strategy that enhances biodegradative potential via dissemination of degradative genes from introduced microorganisms to indigenous microorganisms. Bioremediation experiments using 2,4-dichlorophenoxyacetic acid (2,4-D)-contaminated soil slurry and strains of Pseudomonas putida or Escherichia coli harboring a self-transmissible 2,4-D degradative plasmid pJP4 were conducted in microcosms to assess possible effects of gene bioaugmentation on the overall microbial community structure and ecological functions (carbon source utilization and nitrogen transformation potentials). Although exogenous bacteria decreased rapidly, 2,4-D degradation was stimulated in bioaugmented microcosms, possibly because of the occurrence of transconjugants by the transfer of pJP4. Terminal restriction fragment length polymorphism analysis revealed that, although the bacterial community structure was disturbed immediately after introducing exogenous bacteria to the inoculated microcosms, it gradually approached that of the uninoculated microcosms. Biolog assay, nitrate reduction assay, and monitoring of the amoA gene of ammonia-oxidizing bacteria and nirK and nirS genes of denitrifying bacteria showed no irretrievable depressive effects of gene bioaugmentation on the carbon source utilization and nitrogen transformation potentials. These results may suggest that gene bioaugmentation with P. putida and E. coli strains harboring pJP4 is effective for the degradation of 2,4-D in soil without large impacts on the indigenous microbial community. |
| Doi | 10.1007/s10532-011-9505-x |
| Pmid | 21850504 |
| Wosid | WOS:000301792900007 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Gene bioaugmentation; pJP4; Soil slurry; Microbial community structure; Carbon utilization potential; Nitrogen transformation potential |
| Is Qa | No |