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HERO ID
1005052
Reference Type
Journal Article
Title
Toxic effects of lead and nickel nitrate on rat liver chromatin components
Author(s)
Rabbani-Chadegani Iii, A; Fani, N; Abdossamadi, S; Shahmir, N
Year
2011
Is Peer Reviewed?
No
Journal
Journal of Biochemical and Molecular Toxicology
ISSN:
1095-6670
EISSN:
1099-0461
Volume
25
Issue
3
Page Numbers
127-134
Language
English
PMID
21080481
DOI
10.1002/jbt.20368
Abstract
The biological activity of heavy metals is related to their physicochemical interaction with biological receptors. In the present study, the effect of low concentrations of nickel nitrate and lead nitrate (<0.3 mM) on rat liver soluble chromatin and histone proteins was examined. The results showed that addition of various concentrations of metals to chromatin solution preceded the chromatin into aggregation and precipitation in a dose-dependant manner; however, the extent of absorbance changes at 260 and 400 nm was different between two metals. Gel electrophoresis of histone proteins and DNA of the supernatants obtained from the metal-treated chromatin and the controls revealed higher affinity of lead nitrate to chromatin compared to nickel nitrate. Also, the binding affinity of lead nitrate to histone proteins free in solution was higher than nickel. On the basis of the results, it is concluded that lead reacts with chromatin components even at very low concentrations and induce chromatin aggregation through histone-DNA cross-links. Whereas, nickel nitrate is less effective on chromatin at low concentrations, suggesting higher toxicity of lead nitrate on chromatin compared to nickel. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 00:1-8, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20368.
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NAAQS
•
ISA-Lead (2013 Final Project Page)
Considered
Health Effects
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