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Citation
Tags
HERO ID
1021851
Reference Type
Journal Article
Title
Effects of choline on sodium arsenite-induced neural tube defects in chick embryos
Author(s)
Song, G; Cui, Y; Han, ZJ; Xia, HF; Ma, X
Year
2011
Is Peer Reviewed?
Yes
Journal
Food and Chemical Toxicology
ISSN:
0278-6915
EISSN:
1873-6351
Publisher
Elsevier
Volume
Elsevier
Issue
Elsevier
Page Numbers
Elsevier
Language
English
PMID
21708213
DOI
10.1016/j.fct.2011.06.009
URL
https://linkinghub.elsevier.com/retrieve/pii/S0278691511002535
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Abstract
Arsenic passes through the placenta and accumulates in the neuroepithelium of embryo, whereby inducing congenital malformations such as neural tube defects (NTDs) in animals. Choline (CHO), a methyl-rich nutrient, functions as a methyl donor to participate in methyl group metabolism. Arsenic methylation has been regarded as a detoxification process and choline (CHO) is the major source of methyl-groups. However, whether CHO intake reverses the abnormal embryo development induced by sodium arsenite (SA) and the relationship between CHO intake and arsenite-induced NTDs are still unclear. In this study, we used chick embryos as animal model to investigate the effects of SA and CHO supplementation on the early development of nervous system. Our results showed that the administration of SA led to reduction in embryo viability, embryo body weight and extra-embryonic vascular area, accompanied by a significantly increased incidence of the failed closure of the caudal end of the neural tube. CHO, at low dose (25μg/μL), reversed the decrease in embryo viability and the increase in the failed closure of the caudal end of the neural tube, which were induced by SA. In addition, CHO (25μg/μL) inhibited not only the SA-induced cell apoptosis by up-regulating Bcl-2 level, but also the global DNA methylation by increasing the expressions of DNMT1 and DNMT3a. However, less significant difference was found between the embryos co-treated with SA and CHO (50μg/μL) and the ones treated with SA alone. Taken together, these findings suggest that low dose CHO could protect chick embryos from arsenite-induced NTDs by a possible mechanism related to the methyl metabolism.
Tags
IRIS
•
Arsenic Hazard ID
1. Initial Lit Search
PubMed
4. Considered through Oct 2015
6. Cluster Filter through Oct 2015
•
Arsenic (Inorganic)
1. Literature
PubMed
5. Susceptibility Screening
Excluded/Not relevant
•
Arsenic Susceptibility
1. Susceptibility Literature Screening
Keyword Search
2. Excluded
Not Relevant
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