US EPA

1038098 

Journal Article 

Methanol/sorbitol co-feeding induction enhanced porcine interferon-α production by P. pastoris associated with energy metabolism shift 

Gao, MJ; Li, Z; Yu, RS; Wu, JR; Zheng, ZY; Shi, ZP; Zhan, XB; Lin, CC 

2012 

Yes 

Bioprocess and Biosystems Engineering
ISSN: 1615-7591
EISSN: 1615-7605 

35 

7 

1125-1136 

English 

22349926 

10.1007/s00449-012-0697-1 

WOS:000307514500009 

http://dx.doi.org/10.1007/s00449-012-0697-1
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The production of porcine interferon-α (pIFN-α) by Pichia pastoris was largely enhanced when adopting sorbitol/methanol co-feeding induction strategy at 30 °C in a 10-L fermentor. Analysis of energy regeneration pattern and carbon metabolism revealed that major energy metabolism energizing pIFN-α synthesis shifted from formaldehyde dissimilatory energy metabolism pathway to TCA cycle under the methanol/sorbitol co-feeding induction strategy. The sorbitol/methanol co-feeding induction strategy weakened formaldehyde dissimilatory pathway and repressed the accumulation of toxic metabolite-formaldehyde, reduced theoretical oxygen consumption rate and oxygen supply requirement, and increased energy/methanol utilization efficiency so that more methanol could be effectively used for pIFN-α synthesis. As a result, pIFN-α antiviral activity reached a highest level of 1.8 × 10(7) IU/mL which was about 10- to 200-folds of those obtained under pure methanol induction at 20 and 30 °C, respectively. 

Co-feeding; Energy metabolism; Induction; Pichia pastoris; Porcine interferon-alpha