US EPA

1039192 

Journal Article 

Biological evaluation of cobalt(II) complexes with non-steroidal anti-inflammatory drug naproxen 

Dimiza, F; Papadopoulos, AN; Tangoulis, V; Psycharis, V; Raptopoulou, CP; Kessissoglou, DP; Psomas, G 

2012 

Yes 

Journal of Inorganic Biochemistry
ISSN: 0162-0134
EISSN: 1873-3344 

107 

1 

54-64 

English 

22178666 

10.1016/j.jinorgbio.2011.10.014 

WOS:000299361900008 

Cobalt(II) complexes with the non-steroidal anti-inflammatory drug naproxen in the presence or absence of nitrogen-donor heterocyclic ligands (pyridine, 2,2'-bipyridine or 1,10-phenanthroline) have been synthesized and characterized with physicochemical and spectroscopic techniques. The deprotonated naproxen acts as monodentate ligand coordinated to Co(II) ion through a carboxylato oxygen. The crystal structure of [bis(aqua)bis(naproxenato)bis(pyridine)cobalt(II)], 2 has been determined by X-ray crystallography. The EPR spectrum of complex 2 in frozen solution reveals that it retains its structure. UV study of the interaction of the complexes with calf-thymus DNA (CT DNA) has shown that the complexes can bind to CT DNA and [(2,2'-bipyridine)bis(methanol)bis(naproxenato)cobalt(II)] exhibits the highest binding constant to CT DNA. The cyclic voltammograms of the complexes recorded in DMSO solution and in the presence of CT DNA in 1/2 DMSO/buffer (containing 150 mM NaCl and 15 mM trisodium citrate at pH 7.0) solution have shown that they can bind to CT DNA by the intercalative binding mode which has also been verified by DNA solution viscosity measurements. Competitive study with ethidium bromide (EB) has shown that the complexes can displace the DNA-bound EB indicating that they bind to DNA in strong competition with EB. Naproxen and its cobalt(II) complexes exhibit good binding propensity to human or bovine serum albumin proteins having relatively high binding constant values. The antioxidant activity of the compounds has been evaluated indicating their high scavenging activity against hydroxyl free radicals and superoxide radicals. 

Non-steroidal anti-inflammatory drugs; Naproxen; Cobalt(II) complexes; Interaction with calf-thymus DNA; Interaction with albumins; Free radical scavenging activity