Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
1043501
Reference Type
Journal Article
Title
Preparation and PEGylation of exendin-4 peptide secreted from yeast Pichia pastoris
Author(s)
Zhou, J; Cai, ZH; Li, L; Kou, C; Gao, YF
Year
2009
Is Peer Reviewed?
1
Journal
European Journal of Pharmaceutics and Biopharmaceutics
ISSN:
0939-6411
EISSN:
1873-3441
Volume
72
Issue
2
Page Numbers
412-417
Language
English
PMID
19602371
DOI
10.1016/j.ejpb.2009.02.001
Web of Science Id
WOS:000267000800017
URL
http://linkinghub.elsevier.com/retrieve/pii/S0939641109000447
Exit
Abstract
Exendin-4, a peptide analogue of glucagon-like peptide (GLP-1), has been developed for treatment of type 2 diabetes. Herein, the secretive exendin-4 peptide, expressed by methanol induction in Pichia pastoris, was purified to near homogeneity by Ni-NTA agarose chromatography. 103.6 mg of protein was obtained from 1 L of the supernatant and its purity was 96.1%. Subsequently, the PEGylated exendin-4 was prepared. The bioactivity of exendin-4 was determined by examining the glucose-lowering and insulin-releasing ability in plasma. Then, a safety evaluation was performed by histological examination of the main organs (liver, kidney and pancreas). PEGylated exendin-4 displayed glucose-lowering and insulin-stimulating action in vivo without obvious damage to the above organs. The results suggest that the P. pastoris expression could be used to produce large quantities of exendin-4 and PEGylation is a useful tool to maintain and enhance bioactivity of the peptide.
Tags
IRIS
•
Methanol (Non-Cancer)
Search 2012
ProQuest
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity