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1048758 
Journal Article 
Review 
Expression of recombinant proteins in Pichia pastoris 
Li, P; Anumanthan, A; Gao, XG; Ilangovan, K; Suzara, VV; Düzgüneş, N; Renugopalakrishnan, V 
2007 
Yes 
Applied Biochemistry and Biotechnology
ISSN: 0273-2289
EISSN: 1559-0291 
Humana Press Inc., 999 Riverview Dr., Ste. 208 Totowa NJ 07512 USA, [mailto:jchasse@humanapr.com], [URL:http://humanapress.com] 
142 
2 
105-124 
English 
18025573 
WOS:000250070000001 
Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode. 
Pichia pastoris; protein expression; methanol induction; dissolved oxygen; gene integration; alcohol oxidase promoter; AOX1 
IRIS
• Methanol (Non-Cancer)
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