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1051983 
Journal Article 
Analysis of hallucinogenic constituents in Amanita mushrooms circulated in Japan 
Tsujikawa, K; Mohri, H; Kuwayama, K; Miyaguchi, H; Iwata, Y; Gohda, A; Fukushima, S; Inoue, H; Kishi, T 
2006 
Yes 
Forensic Science International
ISSN: 0379-0738
EISSN: 1872-6283 
164 
2-3 
172-178 
English 
16464551 
WOS:000242542700016 
The constituents of seven mushrooms sold as Amanita muscaria or Amanita pantherina (five A. muscaria and two A. pantherina) and four "extracts purported to contain A. muscaria" products that are currently circulated in Japan were determined. All mushroom samples were identified as A. muscaria or A. pantherina by macroscopic and microscopic observation. The dissociative constituents, ibotenic acid (IBO) and muscimol (MUS), were extracted with 70% methanol twice and determined by gas chromatography/mass spectrometry. The IBO (as the hydrate)/MUS contents were in the range of <10-2845ppm/46-1052ppm in the cap of A. muscaria and 188-269ppm/1554-1880ppm in the cap of A. pantherina. In the caps, these compounds had a tendency to be more concentrated in the flesh than in the cuticle. On the other hand, the IBO/MUS contents in the stem were far lower than in the caps. In the "extracts purported to contain A. muscaria" products, IBO/MUS were detected below the lower limit of calibration curve (<10ppm/<25ppm) or not detected. However, these samples contained other psychoactive compounds, such as psychoactive tryptamines (5-methoxy-N,N-diisopropyltryptamine and 5-methoxy-N,N-dimethyltryptamine), reversible monoamine oxidase inhibitors (harmine and harmaline) and tropane alkaloids (atropine and scopolamine), which were not quantified. This is the first report of the chemical analysis of Amanita mushrooms that are circulated in the drug market. 
Amanita muscaria; Amanita pantherina; ibotenic acid; muscimol; GC/MS 
IRIS
• Methanol (Non-Cancer)
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